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After incubation, 1 mL of absolute EtOH was added and SP3 beads were magnetically captured and washed three times with 80% EtOH, then resuspended in 200 L 50 mM HEPES buffer (pH 7.4) containing 1 mM TCEP and incubated for 3 min at RT
doi: 10.3390/ani14010138 63
Tom D, Almeida RD
& Stow, J
The glutathionylated precursor of 3MH comes from the natural breakdown of fatty acids in the endosperm and germ of the barley
