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5.Refrigerate immediately after reconstitution

Lancet 374 , 3947 (2009)

Since the enzyme catalyzing the first reaction in a pathway is often the regulatory enzyme, this prevents: the synthesis of unneeded intermediates that would occur if regulation happened downstream

This can occur when enough proteins belonging to a specific pathway differ between groups, resulting in a significant enrichment score, but the pattern of protein expression is inconsistent with one direction of pathway activity or there is not enough data to predict an activation z-score

A.PapavassiliouA

The role of gravity cannot be overlooked either