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Among them, 5-oxoproline, an intermediate in the biosynthesis of the endogenous glutathione (GSH), was increased

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This experimental model was used to assess the leukocyte expression patterns of 11 biomarkers previously associated with VCD in long COVID and explore the potential of pharmacological B12 in regulating these genes

For solubilizing these polypeptides, variables such as non-denaturing detergents (IGEPAL CA-630), changing the ionic strength of the cell lysis and solubilization buffer, addition of BugBuster, diluting the cell lysate and sonication were introduced