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With that test, Ames and other investigators were able to show that most cancer-causing chemicals act by damaging genesa finding that now seems obvious only because Ames helped prove it

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However, high doses of caffeine can cause adverse effects like a fast heartbeat, trouble sleeping, jitteriness, and anxiety

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Animals were acclimatized for one week before the experiments and maintained under standard laboratory conditions (22 2 C, 55 5% relative humidity, 12-h light/12-h dark cycle) with ad libitum access to standard pellet chow and water