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13 C NMR (101 MHz, CDCl 3 ) 159.22, 157.30, 151.70, 143.94, 141.78, 140.58, 137.89, 136.38, 132.42, 130.29, 129.82, 129.73, 129.64, 129.35, 128.75, 128.64, 127.23, 126.94, 118.57, 113.41, 73.36, 55.32, 46.44, 35.27, 28.94, 19.91

However, patience is key, as initial darkening may occur before lightening, as explained in the product guidelines

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3.5 Age-related changes in other glial cells WM glial cells, including microglia and astrocytes, are also critical for the normal function of WM

Afterwards (D3-D4), cells were split using the Trypsin/EDTA solution (BioWest) and seeded for experiments