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Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin

KPV: Anti-Inflammatory Power Without Immunosuppression Mechanism of Action: KPV is a tripeptide fragment (Lys-Pro-Val) of alpha-melanocyte-stimulating hormone (-MSH)

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GHK-Cu, TB-500 and BPC-157 are experimental peptides and are not approved for human use in the European Union, the United States, or most other jurisdictions

An internal laqI q gene helps to maintain tight control over expression of the insert by binding to the tac promoter until induction with IPTG

Cayrol C, Knibiehler M, Ducommun B