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RNA isolation and real-time qPCR analysis Total RNA was isolated using the FastPure Cell/Tissue Total RNA Isolation Kit V2 (Vazyme, RC112, Nanjing, China) according to the manufacturers instructions

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Consistent with this observation, treatment with the specific APT1 inhibitor ML348 resulted in increased S-palmitoylation of ATG2A, whereas the specific APT2 inhibitor ML349 produced only a minimal effect (Fig

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[11] Age may affect the bodys production of glutathione