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Description
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Samples were centrifuged at 3,000 g for 10 min at 4C to create a gradient, followed by removal of the top clear layer and middle debris-containing layer (45 ml)

doi: 10.1080/07315724.1998.10720458

Moreover, it can induce the production of urea, thereby reducing the concentration of ammonia and improving the nervous function of the body [253]

It also decreased oxidative stress and inflammatory cytokine production in the DED model
