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coli : Escherichia coli FDR: False discovery rate, the fraction of positive reports that are incorrect GAL1 promoter: Galactose-inducible promoter sequence (originating from the GAL1 gene) GBT: Gradient-boosted trees, a machine learning algorithm gnomAD: Genome Aggregation Database LC-MS/MS: Liquid chromatography followed by tandem mass spectrometry MAF: Minor allele frequency, the frequency of a given minor allele MAT cys4::KanMX his31 leu20 lys20 ura30 : Yeast strain of mating type alpha, carrying a kanamycin resistance cassette that replaces the CYS4 locus, as well as carrying null alleles for LEU2 , LYS2 and URA3 , which convey auxotrophies to leucine, lysine, and uracil mRNA: Messenger ribonucleic acid NGS: Next-generation sequencing technology NMD: Nonsense-mediated decay NNK: Degenerate codon consisting of two random nucleotides (A/C/G/T) followed by a single keto-nucleotide (G/T) N-terminus: Amino-group terminus of a protein sequence OMIM: Online Mendelian Inheritance in Man, a database of Mendelian disorders ORF: Open reading frame, a nucleotide sequence that stretches form a start to a stop codon

Testing follows USP , ICH Q3E guidelines, and IPAC-RS best practices for OINDPs, using LC-MS/MS and GC-MS/MS to identify and quantify unknown migrated compounds

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