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The observed effects are utilised to establish the chemical's lethal concentration (LC 50 ), defined as the concentration at which fifty per cent of the exposed fish die (Xiong et al., 2022)

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doi: 10.1101/gad.2016111 245 YangARajeshkumarNVWangXYabuuchiSAlexanderBMChuGCet al

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After that, 10 studies were excluded (they did not meet our inclusion criteria, and two not full text)