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the cells were washed with PBS and NR detainer solution (50% ethanol, 49% deionized water, 1% glacial acetic acid) was added, and the plate was shaken rapidly on a microtiter plate until a homogenous solution was reached the plate was transferred to a plate reader at =570 nm and the absorbance was measured with a microplate reader (EPOCH, BioTek Instruments, VT, USA)

These data imply that in the mammalian axons of the adult brains, presynaptic mitochondria are probably not the main source of ATP

CBS activity increases the flux via the transsulfuration pathway, which leads to GSH production, which becomes oxidized and depleted during oxidative stress