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[PMC free article: PMC3183054] [PubMed: 21980433] 101

Epigenetic modification of Nrf2 in 5-fluorouracil-resistant colon cancer cells: involvement of TET-dependent DNA demethylation

The reduction in absorbance at 560 nm in the presence of antioxidants indicates the neutralization of superoxide anions in the reaction mixture (Bursal and Glin 2011)

The benefits vary by target application, and each has distinct evidence behind it

Weekly dosing helps keep levels more stable over time

Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively