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While many tumor cells exhibit the Warburg effect, 260 which favors aerobic glycolysis, the metabolic profiles of CSCs are far more diverse and adaptive, often reflecting the unique demands of maintaining a stem-like state in microenvironments

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Knockdown of Chac2 (Sh-Chac2) via lentiviral transduction significantly reduced its expression at both the mRNA and protein levels, as confirmed by RT-qPCR and Western blot (Fig