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2.2 Add Monochlorobimane working solution and incubate at 37C for 30 minutes (optimized according to the experiment)

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The earliest step of GSH biosynthesis consists of a reaction between the -carboxyl group of Glu and -amino group of Cys to create an amide bond to yield -ECS

Life Sci (2005) 77(15):183643.10.1016/j.lfs.2005.02.014 97 IsobeCAbeTTerayamaY