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if your calculated dose volume is larger than 30 units, you'd need to use two syringes, which isn't ideal

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10.1172/JCI7013

The formation of fibrillar aggregates was characterized by the half-time of aggregation (T), defined as the time required to reach 50% of the maximum Th-T fluorescence intensity, as derived from fitting the kinetic curves to a one-phase exponential association model

HIS fusion proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen), and GST fusion proteins were purified with Pierce glutathione agarose (Thermo Scientific) according to the standard protocols provided by the manufacturers