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Post-operatively the animals were housed individually in an American Accreditation for Laboratory Animal Care-approved vivarium maintained at 221 C on a 12-h alternating light/dark cycle initiated at 06:00 h
Filtration: Filtration After mixing, sterile solution is subjected to filtration, which is crucial to retain the sterility of formulation
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Sections of the brain and other major organs were rapidly frozen in liquid nitrogen and stored at -80C
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