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Band 3 is present in over a million copies per cell and has important functions concerning ion exchange and mechanical support (7, 27)

Sucrose fractionation Quadriceps (2.4 mg) pooled from five animals of each group were homogenized with a Dounce homogenizer in 1 mL polysome extraction buffer (PEB): 20 mM TrisHCl pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3 % NP-40, 60 U/mL RiboLock RNase Inhibitor (Thermo Fisher Scientific), 100 g/mL cycloheximide, 5 M FAD, cOmplete EDTA-free protease inhibitor cocktail and phosphatase inhibitor cocktail (Sigma-Aldrich)

You will thus benefit from all the protective and detoxifying effects of Glutathione with the assurance of effective doses

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doi: 10.1074/jbc.M513187200 [DOI] [PubMed] [Google Scholar] 416

92 Knave B, Persson HE, Goldberg JM, Westerholm P