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Secondary antibodies included Secondary donkey IgG directed against goat IgG conjugated with Cy5(Jackson ImmunoResearch Labs Cat# 705-175-147, RRID: AB_2340415), Secondary donkey IgG directed against rabbit IgG conjugated with Cy3(Jackson ImmunoResearch Labs Cat# 711-165-152, RRID: AB_2307443) and Secondary donkey IgG directed against mouse IgG conjugated with 488(Jackson ImmunoResearch Labs Cat# 715-545-150, RRID: AB_2340846), both at a dilution of 1:200 (Molecular Probes, Eugene, OR, USA)