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Yeast Neutral Comet Assay For yeast neutral comet assay 10 5 cells were collected, centrifuged and washed with S-buffer (1 M sorbitol, 25 mM KH 2 PO 4 , pH 6.5)

For these two EGT treatments, GSH concentrations declined 46% to 49% over the 10-day storage period

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This actin-buffering capacity is critical for dynamic cellular processes including: Cell migration: Rapid actin polymerization at the leading edge Morphological changes: Cytoskeletal reorganization Intracellular transport: Actin filament dynamics The peptide contains a conserved LKKTETQ actin-binding motif that is essential for its interaction with G-actin