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Sci Rep (2022) 12(1):20912

ROS and nitrogen species are synthesized at low levels in skeletal muscle cells and are required for normal force production

Third, TMAO increases circulating pro-inflammatory chemokines and cytokines, which are known to lead to the progression of heart failure [1, 47, 48]

Modified Gomori trichrome staining Consecutive 12-m sections of unfixed fresh-frozen muscle (WT, Alix / , and ccOzz / ) were prepared on a cryostat, stained with Harris hematoxylin for 5 min, washed with water, and then stained with modified Gomori trichrome stain (0.6 g chromotrope 2R, 0.3 g Fast Green FCF, 0.6 g phosphotungstic acid, 1 mL glacial acetic acid in 100 mL deionized water)

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