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As this type of works become commonplace, we believe that SNSPDs can be developed as more practical units since there are no physical or technological limitations

PCR reactions were carried out using 0.5 L of gene-specific cDNA samples and that of 16S rRNA as templates with 1 mM final concentration of each of the forward and reverse RT primers (Additional file 12: Table S5) and 4 L of 2 SYBR green PCR mix in a final volume of 10 L

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Although all subjects took the study drug in the morning, the patterns seen for enclomiphene citrate were best characterized by a mid-day trough with relatively higher levels in the morning and evening for each individual

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