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& Gower, D

doi: 10.1016/j.tox.2023.153580 2

However, the levels of other metabolites such as 5-Aminolevulinate, cysteate, putrescine, 4-Aminobutyraldehyde, kynurenine, serotonin, Formyl-5-hydroxykynurenamine, 5-Hydroxykynurenine, 2-Aminomuconate, xanthine, and 5-Aminolevulinate were significantly increased in LC patients ( Figure 3C , Supplementary Figure 3A )

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Following the removal of the DTT solution, alkylation was performed by incubating the samples with 25 mM iodoacetamide (IAA) in the dark at room temperature for 30 min
