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Critical roles of isoleucine-364 and adjacent residues in a hydrophobic gate control of phospholipid transport by the mammalian P4-ATPase ATP8A2

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Of note, lipid-bound Atg8, which inserts into the membrane of autophagosomes and is important for their formation, can be expressed as a green fluorescent protein (GFP)-tagged protein for use as a marker of autophagosome abundance 29