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ROS generation in cells was assessed using the probe 2,7-dichlorofluorescein (DCF) (Molecular Probes, Eugene, OR, U.S.A.)

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Proteins were transferred to PVDF membranes (Millipore, Billerica, MA, USA)

PMCID: PMC2696075

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The phosphorylation of histone H2AX was detected to evaluate the DNA damage related to behavioral activation therapy (BA) treatment, and CHE was further reported to increase the expression of gH2AX and regulate caspase-3 cleavage at different levels (Hammerov et al., 2011)