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T1DM is also associated with an increased risk of comorbidities, such as cardiovascular disease, retinopathy, and diabetic kidney disease (DKD), further contributing to the burden of this disease

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4.9.1 SDS-PAGE preparation and western blotting analysis Red blood cell plasma membranes were heated for 10 min at 95 C after dissolving in Laemmli buffer (Laemmli, 1970)

Trypsin solution was added to the collagenase-digested tumor tissue, shaken on a shaker for 10 min, and fetal bovine serum was added to terminate the digestion

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