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doi: 10.1136/thx.48.10.985

coli (OD 600 0.5) were washed twice with sodium phosphate buffer (100 mM Na-phosphate buffer (Na 2 HPO 4 /NaH 2 PO 4 ), 0.5 mM EDTA, pH 7.5) and lysed using a triple freezing procedure (liquid nitrogen, 196C), defrosting (37C)

Palomba E, Martone F, Meduri A, Vaccaro A, Damiani N: Biochemical studies on equine infectious anaemia