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Clinical markers of progression Older age, male sex, higher baseline motor scores and worse baseline cognition have all been linked to worse motor and cognitive outcomes at longitudinal follow-up 141,182,194,195,196 and have been integrated with sleep disorder, presence of depression and various genetic, imaging or CSF biomarkers into clinical predictive scores 182,196

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An exhaustive list of all parameters detected can be find in Annex 1

Targeting oxidative stress in central nervous system disorders

In each condition, 200 ng of the 5XUAS-tk-luc reporter was transfected, together with 25 ng of the pCMV-Gal4- Dmrta2 or pCMV-Gal4 and different doses of pCDNA3-Myc- Zfp423 (200, 400, and 600 ng) expression plasmids

TRIzol reagent and PCR primers were provided by Invitrogen (Carlsbad, CA, USA)