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Note that ascites was not present in all mice

Caked or clumped powder has likely absorbed humidity and begun degrading even before reconstitution

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Furthermore, ROS also activates various enzymes, including phospholipase A, thereby destroying the integrity and permeability of the cell membrane of the nervous system, leading to cell death and reperfusion injury (Chen et al., 2011

A significant increase in serum levels of the inflammatory factors IL-1B, TNF-a, and IL-6 was found in hemoglobin-injected mice, whereas the use of inflammatory vesicle inhibitors significantly reduced the levels of hemoglobin-induced inflammatory factors
