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Cells were fixed with 4% PFA in PBS at room temperature for 15 min, permeabilized with 1% saponin and stained by rabbit anti-ACP1 antibody (LifeSpan Bioscience) followed by Alexa 488-conjugated goat anti-rabbit IgG antibody (Invitrogen)

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doi: 10.4093/dmj.2013.37.1.63 58 ZhangYChenX

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Record what you told the patient, why you made your clinical decision, and any referrals you suggested

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