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With persistent hypoxia, HIF-1 remains activated and drives abnormal expression of downstream genes, including heme oxygenase-1 ( H O -1 ) (Zhai et al., 2023)

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Additionally, the quality of RNA was determined using High Sensitivity RNA ScreenTape (5067-5579, Agilent) for the TapeStation (Agilent)

Abstract Strategies for the biological repair of intervertebral discs derive from the premise that disc degeneration results from impaired cellular activity and, therefore, that these structures can be induced to regenerate by implanting active cells or providing factors that restore normal cellular activity

[79] showed that TGF- functions as a down-regulator of the expression of the enzyme GCL, the rate-limiting step enzyme involved in the synthesis of GSH as mentioned earlier, which negatively effects the production of GSH