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Enzyme activity was calculated using NADPHs extinction coefficient, which was 6.22 mM 1 cm 1
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The mechanism of superoxide production by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria

For quantitation of mitochondrial potential and mass of FoB and MZB, splenocytes were stained with 100 nM MitoTracker Deep Red FM and 10 nM MitoTracker Green FM (Fisher Scientific 15754272 and 15784272) as follows

66 During the S phase, ROS modulate CDK2-cyclin A activity and intra-S phase checkpoints, with mitochondrial ROS accelerating replication onset

Nanomedicine 11, 17591778
