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Following a centrifugation at 2,500 rpm for 10 min, the upper layer (water layer) was carefully removed and discarded

Therefore, the sulfuranyl free radical on the S-atom of Met35 could be formed by molecular oxygen interaction with the S-atom of Met35, leading to an electron transfer to oxygen from the S-atom resulting in the reduction of oxygen to superoxide free radical and the one-electron oxidation of the S-atom to the sulfuranyl free radical and/or ii ) Cu 2+ weakly bound to the S-atom of Met35 conceivably would lead to a one-electron reduction of Cu 2+ and oxidation of the S-atom to the sulfuranyl free radical as discussed above

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If fatty acid oxidation is defective, fat cannot be utilized, glucose is consumed without regeneration via gluconeogenesis (due to inhibition of pyruvate carboxylase), ketones cannot be produced and there is a drop in glucose levels (hypoglycemia)