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As an alternative approach to measure -arrestin recruitment, bioluminescent resonance energy transfer (BRET) was performed

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Complex purification Cell pellet was thawed in 20 mM HEPES pH 7.4, 50 mM NaCl, 5 mM CaCls, 2 mM MgCl 2 supplemented with cOmplete Protease Inhibitor Cocktail tablets (Roche) and benzonase (Merk Millipore)

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