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The double substitution of L232 3.35b /M233 3.36b with V/T reduced affinity by 10-fold and potency by 100-fold (Moon et al., 2012), whereas the single substitution of M233 3.36b with A and T abolished GLP-1 affinity and potency (Yang et al., 2016)

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Notably, while GLP-1 can potentially affect glucagon secretion via its stimulatory effect on the -cells in normal physiological conditions, GLP-1 also inhibits glucagon release in type 1 diabetes, thus demonstrating that GLP-1 inhibition of glucagon secretion does not fully depend on the -cell secretome [516]

To explore the effect of ghrelin and GLP-1 on the cellular excitability of Glp1r + Ghsr + neurons, we studied these neurons intracellular signalling mechanisms