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Peptide-based drugs often require injection because: They degrade easily outside controlled environments They are too large to cross intact skin barriers Stable blood levels are difficult to maintain via topical application The Johns Hopkins Medicine resource on obesity management reinforces that medically supervised pharmacotherapy is part of comprehensive care rather than a standalone quick fix

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Mitochondrial labelling The Mitotracker-red (M22425, Thermo) and Mitotracker-green (M7514, Thermo) dyes were used to stain mitochondria in living cells, which identify healthy (Mitotracker-red) and total (both healthy and unhealthy Mitotracker-green) mitochondria

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