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The Mytho gene was also subcloned in the pEGFP-N3 vector (4.7 kb) (Addgene) with KOD Hot Start DNA polymerase using the following primers with sticky ends: Fw 5-AAAGCTAGCATGCTGGACCTGGAGGTGGT-3 and Rw compl

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Following staining, cells were acquired on a BD FACS Symphony and analyzed using FlowJo V10

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