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Furthermore, in ECL1 of GCGR there are two glycine residues (G207 ECL1 , G219 ECL1 ) that disconnect the helical structure in the active state, whereas the G219 ECL1 is not conserved in GLP-1R and the helical conformation is relatively intact in this region of GLP-1R

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The fluorescence measurements were performed using a FLUOstar Omega (BMG Labtech) plate reader, with an excitation filter at 355 nm and an emission filter at 482 nm, at a gain of 500 and 8 flashes per well

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