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d) condensing Fmoc-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-OH (fragment-2) with 20 amino acid peptide chain obtained in step-c) in presence of a coupling agent followed by deprotection in presence of a base to obtain 27 amino acid peptide chain

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