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Available from: Accessed June 1, 2025

Human embryonic kidney 293 cells containing SV40 large T-antigen (HEK293T) were cultured in DMEM (Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco), 1 mM sodium pyruvate (Gibco) and 100 units/mL penicillin and 100 g/mL streptomycin at 37 C in 5% CO 2

Since obesity can cause chronic inflammation, including inflammation in the brain, it can also increase the risk of developing neurodegenerative diseases such as Alzheimers disease