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NAFLD Activity Score, fibrosis stage and histological assessment of steatosis, inflammation, and fibrosis Paraffin embedded slides of biopsied baseline liver tissue (50 to 100 mg) and terminal samples (approximately 200 mg) were prepared to assess hepatic morphology including steatosis and fibrosis by staining with hematoxylin and eosin (H&E) and picrosirius red, respectively