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The liver extensively metabolizes it into glycine and taurine conjugates
sgRNAs against exons 4 and 5 of the Gipr , Glp-1r and Gcgr genes (ESM Table 2) were designed using CRISPOR tool (www.crispor.tefor.net), in vitro transcribed (E3322, NEB) and purified using the MEGAclear Kit
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Cell viability assay Cell viability was tested using CCK-8 kit (Dojindo, Japan) according to the its protocol
