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Patients utilizing them 75 to 100 percent of the time have greater exposure to drug-specific issues
Quantitative PCR Total RNA was isolated from MIN6 cells using RNAiso Plus reagent (Takara Bio Inc., Shiga, Japan), and 2-g aliquots were converted into cDNA using an Applied Biosystems High Capacity cDNA Reverse Transcription Kit (Life Technologies Co, Carlsbad, CA)
Snell-Bergeon JK, Kaur G, Renner D, Akturk HK, Beatson C, Garg SK
The results demonstrated clear superiority over placebo across all measured endpoints
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