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2.8 Real-time quantitative PCR Total RNA was isolated from BMSCs cultivated under various conditions employing TRIzol reagent (Ambion, United States), and cDNA was synthesised employing the cDNA HiFiScript Synthesis Kit (Cwbio, China)

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Neurotransmitter Balance: Imbalances in neurotransmitters, such as serotonin and dopamine, can contribute to mood disorders and behavioral abnormalities

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