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Media was removed and cells were washed with cAMP stimulation buffer (Hanks balanced salt solution (HBSS), 20 mM HEPES, pH 7.4) and then incubated in cAMP stimulation buffer for 30 min at 37C and 5% CO 2

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The fundamentals, she says, are the same for everyone on the medications or not: enough protein, enough fiber, and nutrient-dense foods that are not overly processed

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