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The dried extract was reconstituted in 1 N HCl solution and hexanes (1:1), transferred to a separatory funnel, and shaken vigorously

A decline in TOC removal efficiency may signal an issue with biofilm activity, such as a disruption in microbial growth or insufficient oxygen levels
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Follow sterile methods using syringes, alcohol swabs, and clean working conditions

Unlike NAD+ precursors that work downstream, 5-Amino-1MQ acts upstream by blocking NNMTs consumption of S-adenosyl methionine (SAM), resulting in elevated intracellular NAD+ and SAM levels without requiring direct nucleotide supplementation
