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A reaction mixture (GSSG) was made by combining 2 mM EDTA, 0.15 mM NADPH, 50 mM buffer solution of potassium phosphate (pH 7.8), 0.2 ml enzyme extract, and 0.5 mM oxidized glutathione
In contrast, other hairs underwent double transitions and reversions, meaning the hair began dark, started to gray, then re-pigmented again

After 30 minutes of incubation, cells were detached with TrypLE (Gibco), spun down and resuspended in PBS
doi: 10.1001/jamacardio.2017.2511 134 LinRRahtu-KorpelaLMaggaJUlvilaJSwanJKemppiAet al
Insulin-like growth factor (IGF-I) improves height in growth hormone insensitivity: two years results

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