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A431, HT-29 and MCF7 cells lines, which express the targeted antigen EGFR, were used as positive cell lines while a CHO cell line was used as a negative control cell line

usually mild-to-moderate and easing with time [2] [4]

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In addition, BDMC suppresses breast cancer (BT-549, MDA-MB-231, and MCF-7) progression through down-regulating cancer metastasis-related proteins (MMP-9 and TWIST1) and inducing pro-apoptotic proteins (p53) expression [404, 405]

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Hypomethylation also leads to the silencing of BRCA1, a major tumor suppressor gene implicated in breast cancer that ordinarily prevents cancer cell growth, leading to resistance to drugs like tamoxifen [102,103]