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Mouse-study observations In the Billon 2023 and Billon 2024 studies, mice dosed at 25-50 mg/kg IP for up to 28 days showed no overt toxicity, no sickness-related weight loss, no liver-enzyme signal at the doses tested, and no cardiac dysfunction

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These included mechanical tissue damage simulations, chemically induced gastric lesions, vascular interruption models, and inflammation-driven structural breakdown in animal subjects

AMPK is activated during the calcium switch-induced assembly of ZO-1, which facilitates tight junction assembly 81

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