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RNA Extraction and RT-PCR in the Tendon-Muscle Junction Samples At 7, 14, 28, and 42 postoperative days, TRIzol Reagent (Thermo Scientific, Waltham, MA, USA) was used for extraction, and spectrophotometer (NanoDrop, Thermo Scientific) for quantification at 260 nm of the tissue RNA (Table 1)

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The fatigue likely stems from multiple factors working together

BPC-157 derived from a gastric protective protein, studied for wound healing and gut protection pathways
