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The sections were then washed three times in TNT buffer (0.1 M TrisHCl, 0.15 M NaCl, 0.05% Tween20, pH 7.5) for 5 min, and treated with secondary antibodies

[DOI] [PubMed] [Google Scholar] 276.Oltman CL

To assess the role of 3-AR in mediating these effects, researchers examined AOD9604 and hGH treatment in 3-KO mice

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This study also identified decreased concentrations of complexes I, III, IV, and V and weakened activities of complexes I and IV [79]

The M0 macrophages were then stimulated in 24-well plates (1 10 5 cells per well) with either IFN- (20 ng/mL, PeproTech) and LPS (100 ng/mL, Merck KGaA, Darmstadt, Germany) to generate M1-like macrophages (M1) or with IL-4 (20 ng/mL, PeproTech) to generate M2-like macrophages (M2), with or without inhibitors, for 24 h